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KMID : 0900920030270020125
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Korean journal of Animal Reproduction
2003 Volume.27 No. 2 p.125 ~ p.133
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Effect of beta-Mercaptoethanol on In Vitro Maturation of Porcine Folliculay Oocytes and Development of Porcine IVM/IVF Embryos
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Han Man-Hee
Lee Kyung-Bon
Park Byung-Kwon
Seo Kil-Woong
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Abstract
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The present study was carried out to examine the effect of beta-Mercaptoethanol (beta-ME) on in vitro maturation (IVM) of porcine follicular oocytes and oxygen concentration with beta-ME on in vitro development (IVD) of porcine IVM/IVF embryos. The results were summarized as follows. 1. The rates of nuclear maturation, penetrated oocytes, polyspermic oocytes, pronucleus formation and mean numbers of the penetrated sperms were not significantly different using NCSU-23 maturation media for 0, 25, 50 and 100 muM beta-ME (P>0.05). 2. The rates of blastocyst formation at day 7 after in vitro fertilization were higher in oocytes matured with 25 muM beta-ME (25.4pm0.9%) than in those matured with 0 (14.5pm1.6%), 50 (17.3pm1.7%) and 100 muM (12.4pm1.3%) (P<0.05). However, no differences ware found in total cell numbers of blastocyst among the treatments. 3. The rates of blastocyst formation at day 7 after in vitro fertilization were higher in the NCSU-23 Culture medium With 25 muM beta-ME (23.6pm2.8%) than in those Cultured With 0 (15.4pm4.4%), 12.5 (17.5pm2.3%) and 50 muM beta-ME (18.6pm2.1%) Under the 5% and 20% O_2 Concentrations (P<0.05). However, no differences was found in total cell numbers of blastocyst among the treatments. These results suggested that the addition of 25 muM beta-ME in the IVM/IVD media were effective on the porcine embryo production. However, the rates of blastocyst formation and total cell numbers of blastocyst at day 7 of porcine IVM/IVF embryos were not significantly different in the NCSU-23 culture medium under 5% and 20% 02 concentrations.
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KEYWORD
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Porcine embryo, IVM/IVF, beta-ME, beta-mercaptoethanol
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